With years of manufacturing experience, Duoning provides customers with 15 L, 30 L, 50 L, 100 L modular-designed pilot-scale system for microbial fermentation, and customized >200 L production-scale fermentation system. The pilot fermentation systems are widely used in the culture of bacteria, cocci, filamentous bacteria, plant cells, yeast, for optimizing the conditions for large-scale production process, or for clinical material manufacturing. The expert team can provide customers with process design, pipeline construction, system integration and manufacturing, control software development and other services.
System Features
? Modular design, space saving, small footprint;
? Sterilization-in-place, easy to use, labor saving;
? Equipped with in-place sampling valve for aseptic sampling;
? The rational use of axial flow impeller and radial flow impeller provides a high oxygen transfer rate to ensure the demand for high-density culture;
? Electric heating for temperature control, achieving rapid heating and cooling, suitable for the production of temperature-induced products;
? Enables remote monitoring and communication;
? Temperature control using jacket and tank internal dual PID control loop, achieving stable temperature control, high accuracy within ±0.1℃;
? Pin type feeding port, easy to use, optional for sterilization-in-place feeding valve group;
? Standard configuration with rotameter, optional air/oxygen mixer and mass flowmeter;
? Air intake/exhaust filter sterilization can be carried out independently to meet the requirements of filter replacement during the culture process;
? Pipeline and frame adopt open design, easy to use and maintain;
? Pipeline adopts 3A standard, comply with GMP and BPE requirements;
? All valves or instruments can be switched between automatic and manual mode with one click control on the main screen;
? Instrument/valve control mode: manual control mode can be selected in the main control screen - you can manually switch the valve or instrument; Or automatic control mode: PID control can be selected
? Dissolved oxygen control: DO can be controlled by selecting AIR/O2 flow rate or stirring speed;
? Multi-user authorization: multi-level user authorization with password protection to avoid process parameters and data been arbitrarily modified;
? Software allows for parameter setting, display control, parameters monitoring, curve analysis, data storage, and output printing;
? Multiple levels of control and viewing authorizations.
Technical parameters
Tank body | Total volume | 15 L | 30 L | 50 L | 100 L |
Working volume | 10 L | 8-22 L | 15-35 L | 25 – 72 L | |
Diameter-height ratio | 1:2.2 | 1:2.2 | 1:2.2 | 1:2.2 | |
Material | 316L SS | ||||
Head plate | 316L SS, 4 pieces | ||||
Manufacturing standard | ASME, PED, GB D1/D2, ISO9001:2000 | ||||
Material 316L stainless steel; Jacket 304 stainless steel Polishing accuracy of the surface in contact with the material Ra<0.4 Design pressure: 0.3 MPa, working pressure: 0.25 MPa Connection configuration: safety glass window, side view window, feed valve group port, foam sensor port, pressure sensor port, in-place sampling port, air intake port, bottom valve, inoculation valve group port, base feed port, safety bursting disc | |||||
Stir | Type | Top stir, mechanical seal, PID control | |||
Display | Digital display, ±1 rpm | ||||
Stir speed | 25~800 rpm, ±1 rpm | ||||
Impeller | Six-blade impeller | ||||
Detection probe | pH | 2~12, PID control, accuracy: ±0.01, correlate with base and acid feed pump | |||
DO | 0～200%, PID control, accuracy: ±1%, correlate with gas supply flow rate and stir speed (optional） | ||||
Liquid level | SS probe, PID control | ||||
Temperature | Pt100, PID Control, Digital display, ±0.1℃ | ||||
Gas control | Flowrate measurement | Thermal mass flow controller (optional) | |||
Gas | Four-gas control system (N2, O2, CO2, Air) , solenoid valve control | ||||
Intake filter | 0.2 μm, PTFE | ||||
Exhaust filter | 0.2 μm, PTFE | ||||
Exhaust condenser | 316L SS | ||||
Pressure regulation | Automatic pressure regulating system, including pressure sensor, and regulating valve | ||||
Liquid feed | Peristaltic pump | Variable speed pump, and flow is accumulated, | |||
Temperature | Control method | PID, Automatic sterilization; The temperature is controlled by the external heater and the cooling water under the control of the control system | |||
Control accuracy | Display accuracy: 0.1℃, control accuracy: ±0.1℃ | ||||
Control range | 5 ℃~65 ℃ above cooling water temperature | ||||
Control system | Siemens programmable logic controllers for industrial use | ||||
Optional configurations | Weighing, tank lid automatic lifting | ||||
Water, electricity, gas requirements | Cooling water pressure: 0.15MPa | ||||
Cooling water temperature: not higher than 30 ℃ | |||||
Input air pressure: 0.2MPa (subject to drying and pre-filtration) | |||||
Input steam pressure: 0.18MPa (must be pre-filtered) | |||||
For more information about custom systems, please contact your local representatives or technical support team of Duoning.
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