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Affinity Chromatography Resin
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Antibody Purification Resin


Affinity chromatography resins are mainly used for the capture and purification of monoclonal antibodies/bispecific antibodies, fusion proteins or other protein molecules with specific binding sites. Considering different purification requirements, Bogen (A Duoning company) provides affinity chromatography media based on polymer hard gel matrix (three series of HP/CP/GP according to pore size) and agarose soft gel matrix.

 

The hard gel chromatography resin developed by Bogen uses high-strength hydrophilic polyacrylate beads as matrix, and adopts unique flexible hydrophilic modification technology to further reduce non-specific adsorption caused by hydrophobic micro surface of hard gel chromatography resins , which helps to improve protein recovery and robustness of purification process.

 

Bogen has developed industry-leading multi-scale micro-nano structure control technology, which can realize the precise regulation of the pore size of the chromatography resin. Based on the size of protein molecular weight, we can provide chromatography resins with different pore sizes:

 

?   HP series affinity chromatography resins: suitable for molecules with MW <200 KD

?   CP series affinity chromatography resins: suitable for molecules with MW 100 KD-300 KD

?   GP series affinity chromatography resins: suitable for molecules or viruses/vaccines with MW > 150 KD

 

Antibody Purification Resin

 

Antibody Purification Resin (GP-ProA) is alkali-resistant affinity chromatography resin developed for purification of monoclonal antibody/bispecific antibody, separating antibody of interest from the impurities through the specific binding between ProA ligand and antibody. This product uses super-macro-porous polyacrylate beads as matrix and alkali-resistant recombinant ProA as binding ligand, featuring high flow rate, low back pressure, and high stability, which are obvious advantages in commercial manufacturing of antibody-based biopharmaceuticals.

 

Antibody Purification Resin (ProG) is affinity chromatography resin specially developed for purification of antibodies from ascites and serum. This product uses agarose-based soft gel beads as matrix and r-ProG as binding ligand, achieving high binding capacity and long-life cycle.

 

Product Features

Product

Antibody purification resin (GP-ProA)

Antibody Purification resin (ProG)

Matrix

Super macroporous polyacrylate hard gel

Agarose soft gel

Ligand

r-Protein A

r-Protein G

Average particle size

70μm

90μm

Ligand density

10mg/mL

5mg/mL

Dynamic capacity

≥40mg/mL

≥30mg/mL

Max flowrate

1500 cm/h

600 cm/h

Max Pressure

1 Mpa

0.3 Mpa

Storage

2-8°C (20% ethanol)

2-8 (20% ethanol)

Applicable range

Monoclonal/bispecific antibodies, Fc fusion protein

Human, mouse, bovine, horse derived IgG

PH Stability

Can withstand 0.5M NaOH cleaning (It is recommended that the cleaning time should not exceed 20 minutes)

3-12 (long term)

2-13 (short term)

 

Case Study

 

Chromogram of mAb purification process by GP-ProA

Antibody Purification Resin

Column size: 0.78cm ID x 10cm

Flowrate: 1 mL/min

Detection wavelength: 280nm

Sample: Clarified mAb cell culture medium

Elution purity: 96.65%

Elution recover rate: 92.51%

Concentration factor: 4-10 x


Ordering Information

 

GP series

Product Name

Part No.

Specification

Product Name

Part No.

Specification

Antibody purification resin (GP-ProG)

BG16-3110-02

50mL

Antibody purification resin (GP-ProA)

BG18-3110-02

50mL

BG16-3110-04

500mL

BG18-3110-04

500mL

BG16-3110-05

1L

BG18-3110-05

1L

BG16-3110-07

10L

BG18-3110-07

10L

BG16-3110-08

20L

BG18-3110-08

20L

 

Agarose series

Product Name

Part No.

Specification

Product Name

Part No.

Specification

Antibody purification resin (ProG)

BG16-0010-02

50mL

Antibody purification resin (ProA)

BG18-0010-02

50mL

BG16-0010-04

500mL

BG18-0010-04

500mL

BG16-0010-05

1L

BG18-0010-05

1L

BG16-0010-07

10L

BG18-0010-07

10L

BG16-0010-08

20L

BG18-0010-08

20L

 

Prepacked column

Product Name

Specification

Applicable   Molecular Weight

Capacity

Max Pressure

Max Flow rate

Application/Feature

Antibody purification column (ProG)

5mL

10mL

≤200KD

30mg/mL

0.3MPa

600cm/h

Used to purify antibodies from ascites and serum

Antibody purification column (GP-ProA)

5mL

10mL

≥150KD

40mg/mL

1MPa

1000cm/h

Used for scale-up study of antibody purification process, high flow rate, low back pressure, high stability

 

 


Relevant Documents

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  • Tel.: +86 021 6434 0155
  • E-Mail: marketing@duoningbio.com
  • Address: Building 30, No. 1525 Minqiang Road, Songjiang District, Shanghai
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