日韩成人一区二区三区在线观看,亚洲爆乳成av人在线视菜奈实,一级特黄aaa大片在线观看成人一级片在线观看,欧美一级a视频在线观看,久久综合狠狠色综合伊人,国产亂倫近親相姦视频,日韩免费无码视频一区二区三区,97久久精品亚洲中文字幕无码,久爱无码精品免费视频,超碰97人人模人人爽人人

Affinity Chromatography Resin
Home > Technology > Chromatography Resin > Affinity Chromatography Resin > His-tag protein purification resin

His-tag protein purification resin


His-tag protein purification resin is specially designed for purification of His-tag fusion proteins, it can capture His-tag fusion proteins from cell lysis/culture media. Depending on expression systems, you can choose products with different conjugated ligand types: IDA, NTA and Plus, among which, IDA and NTA are suitable for purifying His-protein expressed in E. coli cells, and Plus is suitable for purifying extracellularly expressed products from insect and mammalian cells.

 

His-tag protein purification resin (GP-IDA) uses super-macro-porous polyacrylate beads as matrix, iminodiacetic acid (IDA) as chelating ligand, and conjugates Ni2+. This product features low mass transfer resistance and is suitable for capturing His-protein from high viscosity feed, including supernatant of E. coli cell lysis.

 

His-tag protein purification resin (CP-NTA) uses macro-porous polyacrylate beads as matrix and nitrilotriacetic acid (NTA) as chelating ligand, which is resistant to low concentration of reducing agent added in protein solution, including B-mercaptoethanol or dithiothreitol (DTT).

 

His-tag protein purification resin (HP-Plus) uses polyacrylate beads as matrix and adopts unique metal chelating ligand (Plus) from Bogen (A Duoning company), which can stably adsorb Ni2+ without falling off even in solutions containing EDTA; and it can be CIP with NaOH while no need to be re-conjugate Ni2+. This product can greatly save time of sample pretreatment and after-process treatment of resin. It is especially suitable for purification of His-tag proteins expressed and secreted eukaryotic cells, including insect and mammalian cells.

 

Product

His tag protein purification resin

(HP/CP/GP-IDA

His tag protein purification resin

(HP/CP/GP-NTA

His tag protein purification resin

(HP/CP/GP-TED

Matrix

Polyacrylate hard gel

Polyacrylate hard gel

Polyacrylate hard gel

Ligand

IDA

NTA

TED

Average particle size

70 μm

70 μm

70 μm

Dynamic capacity mg/mL

HP-IDA

CP-IDA

GP-IDA

HP-NTA

CP-NTA

GP-NTA

HP-TED

CP-TED

GP-TED

≥35

≥25

≥20

≥20

≥15

≥10

≥20

≥15

≥10

Max flowrate

1500cm/h

1500cm/h

1500cm/h

Max Pressure

1MPa

1MPa

1MPa

Storage

4-30°C (20% ethanol)

4-30°C (20% ethanol)

4-30°C (20% ethanol)

Reducing agent resistance

No

β-Me≤100mM; DTT≤5mM

β-Me≤100mM; DTT≤5mM; TCEP≤5mM

Feature

High protein capacity

Low metal ion leaching rate

Resistant to 10 mM ethylenediaminetetraacetic acid   (EDTA), 0.5M NaOH, and 6M guanidine-HCl for 24 hours; resistant to 500mM imidazole, 100mM ethylenediaminetetraacetic acid (EDTA) for 2 hours

 

Bogen can provide different ligand metal chelating resin with different pore sizes according to customer needs.

 

His tag protein purification resin

 

For different purification requirements, Bogen also provides His-tag protein purification resins based on agarose soft gel matrix. Considering different His protein binding ability and transition metal ion selectivity, we also provide IMAC products without chelated metal ions, users can choose to chelate Cu2+, Zn2+, Co2+ and other transition metal ions.

 

Product Features

Product

His tag protein purification resin (HP/CP/GP-IMAC

His tag protein purification resin (IMAC

Matrix

Polyacrylate hard gel

Agarose soft gel

Ligand

NTA

NTA

Average particle size

70μm

90μm

Max flowrate

1500cm/h

500cm/h

Max Pressure

1MPa

0.3MPa

Storage

4-8(20% ethanol

4-8 (20% ethanol)

Feature

Low metal ions leaching rate, and the type of metal ions   can be freely selected

 

Case Study


Purifying His-fusion protein from supernatant of E.Coli lysate.


His-tag protein purification resin 


Ordering Information


HP series

Product Name

Part No.

Specification

Product Name

Part No.

Specification

His tag protein purification resin (HP-IDA)

BG11-1110-02

50mL

His tag protein purification resin (HP-NTA)

BG11-1111-02

50mL

BG11-1110-04

500mL

BG11-1111-04

500mL

BG11-1110-05

1L

BG11-1111-05

1L

BG11-1110-07

10L

BG11-1111-07

10L

BG11-1110-08

20L

BG11-1111-08

20L

His tag protein purification resin (HP-TED)

BG11-1113-02

50mL

His tag protein purification resin (HP-IMAC)

BG11-1101-02

50mL

BG11-1113-04

500mL

BG11-1101-04

500mL

BG11-1113-05

1L

BG11-1101-05

1L

BG11-1113-07

10L

BG11-1101-07

10L

BG11-1113-08

20L

BG11-1101-08

20L

 

CP series

Product Name

Part No.

Specification

Product Name

Part No.

Specification

His tag protein purification resin (CP-IDA)

BG11-2110-02

50mL

His tag protein purification resin (CP-NTA)

BG11-2111-02

50mL

BG11-2110-04

500mL

BG11-2111-04

500mL

BG11-2110-05

1L

BG11-2111-05

1L

BG11-2110-07

10L

BG11-2111-07

10L

BG11-2110-08

20L

BG11-2111-08

20L

His tag protein purification resin (CP-TED)

BG11-2113-02

50mL

His tag protein purification resin (CP-IMAC)

BG11-2101-02

50mL

BG11-2113-04

500mL

BG11-2101-04

500mL

BG11-2113-05

1L

BG11-2101-05

1L

BG11-2113-07

10L

BG11-2101-07

10L

BG11-2113-08

20L

BG11-2101-08

20L

 

Agarose series

Product Name

Part No.

Specification

Product Name

Part No.

Specification

His tag protein purification resin (IDA)

8G11-0010-02

50mL

His tag protein purification resin (NTA)

BG11-0011-02

50mL

BG11-0010-04

500mL

BG11-0011-04

500mL

BG11-0010-05

1L

BG11-0011-05

1L

BG11-0010-07

10L

BG11-0011-07

10L

BG11-0010-08

20L

BG11-0011-08

20L

His tag protein purification resin (TED)

BG11-0013-02

50mL

His tag protein purification resin (IMAC)

BG11-0001-02

50mL

BG11-0013-04

500mL

BG11-0001-04

500mL

BG11-0013-05

1L

BG11-0001-05

1L

BG11-0013-07

10L

BG11-0001-07

10L

BG11-0013-08

20L

BG11-0001-08

20L

 

His tag protein purification prepacked column

Product Name

Specification

Applicable Molecular Weight

Capacity

Max Pressure

Max Flow rate

Application/Feature

His tag protein purification column (HP-IDA)

5mL

10mL

≤200KD

35mg/mL

1MPa

1000cm/h

For His tag protein purification, high dynamic capacity, high maximum pressure

His tag protein purification column (HP-NTA)

5mL

10mL

≤200KD

20mg/mL

1MPa

1000cm/h

For His tag protein purification, low ligand leaching rate, high maximum pressure

His tag protein purification column (HP-TED)

5mL

10mL

≤200KD

≥20mg/mL

1MPa

1000cm/h

For purification of His-tagged proteins expressed in eukaryotic cells, resistant to a certain concentration of EDTA, directly cleaned with NaOH,   high maximum pressure

His tag protein purification column (GP-IDA)

5mL

10mL

≥150KD

20mg/mL

1MPa

1000cm/h

For purification of His-tagged proteins with extremely large molecular weight, or to capture His-tagged proteins from relatively viscous liquids.

His tag protein purification column (GP-NTA)

5mL

10mL

≥150KD

10mg/mL

1MPa

1000cm/h

For purification of His-tagged proteins with extremely large molecular weight, or to capture His-tagged proteins from relatively viscous liquids, low ligand   leaching rate, high maximum pressure

His tag protein purification column (GP-TED)

5mL

10mL

≥150KD

10mg/mL

1MPa

1000cm/h

Purification of ultra-large molecular weight His-tagged proteins expressed in eukaryotic cells, withstands a certain concentration of EDTA,   can be directly cleaned with NaOH, and has high maximum pressure

His tag protein purification column (IDA)

5mL

10mL

≤200KD

40mg/mL

0.3MPa

600cm/h

For purification of His tag protein, high dynamic capacity

His tag protein purification column (NTA)

5mL

10mL

≤200KD

20mg/mL

0.3MPa

600cm/h

For purification of His tag protein, low ligand leaching

His tag protein purification column (TED)

5mL

10mL

≤200KD

15mg/mL

0.3MPa

600cm/h

For purification of His-tagged proteins expressed in eukaryotic cells. It can withstand a certain concentration of EDTA and can be directly cleaned   with NaOH.

 

 

 


Relevant Documents

Contact Us
  • Tel.: +86 021 6434 0155
  • E-Mail: marketing@duoningbio.com
  • Address: Building 30, No. 1525 Minqiang Road, Songjiang District, Shanghai
Message

Copyright ? Shanghai Duoning Biotechnology Co., Ltd. All Rights Reserved Sitemap | Technical Support: Reanod

WeChat
WeChat

Message-

Duoning Biotechnology

+86 021 6434 0155

在线观看免费观看最新| 毛片免费全部无码播放| 免费无码av一区二区三区| 色噜噜狠狠色综合网| 五月天婷婷精品免费视频| 精品久久久久久无码| 国产农村妇女精品一二区| 无码人妻AⅤ一区二区三区| 中文字幕欧美人妻精品一区| 国产电影无码午夜在线播放| 国产精品一区二区AV| 最新亚洲春色AV无码专区| 久久婷婷五月综合97色| 全免费a级毛片免费看视频免| 亚洲AV乱码一区二区三区按摩| 精品人妻系列无码人妻不卡| 国产伦精品一区二区三区| 亚洲免费毛片视频 | 国产人成视频在线免费观看| 中文字幕乱人伦一区二区| 看免费视频一区二区三区| 久久久性色精品国产免费观看| 亚洲色婷婷开心综合久久一区| 国产精品无码免费专区午| 免费看成人毛片无码视频| 日韩无毒视频在线观看| 精品国产AⅤ一区二区三区4区| 无码人妻AⅤ一区二区三区日本| 久久婷婷五月综合色奶水99啪| 全免费A级毛片免费看网站| 国产色又爽视频免费播放| 囯产亚洲精品AA片 | 开心婷婷五月激情综合社区| 女生主动让男生舔自己的坤坤| 欧美日韩国产码高清综合人成| 国产丝袜无码一区二区视频| 久久精品噜噜噜成人AV | 波多野结衣高清无码中文字幕| 国产在线aa视频免费观看| 国产美女裸体丝袜喷水视频| 国产AV无码专区亚洲AV手机麻豆|