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Affinity Chromatography Resin
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His-tag protein purification resin


His-tag protein purification resin is specially designed for purification of His-tag fusion proteins, it can capture His-tag fusion proteins from cell lysis/culture media. Depending on expression systems, you can choose products with different conjugated ligand types: IDA, NTA and Plus, among which, IDA and NTA are suitable for purifying His-protein expressed in E. coli cells, and Plus is suitable for purifying extracellularly expressed products from insect and mammalian cells.

 

His-tag protein purification resin (GP-IDA) uses super-macro-porous polyacrylate beads as matrix, iminodiacetic acid (IDA) as chelating ligand, and conjugates Ni2+. This product features low mass transfer resistance and is suitable for capturing His-protein from high viscosity feed, including supernatant of E. coli cell lysis.

 

His-tag protein purification resin (CP-NTA) uses macro-porous polyacrylate beads as matrix and nitrilotriacetic acid (NTA) as chelating ligand, which is resistant to low concentration of reducing agent added in protein solution, including B-mercaptoethanol or dithiothreitol (DTT).

 

His-tag protein purification resin (HP-Plus) uses polyacrylate beads as matrix and adopts unique metal chelating ligand (Plus) from Bogen (A Duoning company), which can stably adsorb Ni2+ without falling off even in solutions containing EDTA; and it can be CIP with NaOH while no need to be re-conjugate Ni2+. This product can greatly save time of sample pretreatment and after-process treatment of resin. It is especially suitable for purification of His-tag proteins expressed and secreted eukaryotic cells, including insect and mammalian cells.

 

Product

His tag protein purification resin

(HP/CP/GP-IDA

His tag protein purification resin

(HP/CP/GP-NTA

His tag protein purification resin

(HP/CP/GP-TED

Matrix

Polyacrylate hard gel

Polyacrylate hard gel

Polyacrylate hard gel

Ligand

IDA

NTA

TED

Average particle size

70 μm

70 μm

70 μm

Dynamic capacity mg/mL

HP-IDA

CP-IDA

GP-IDA

HP-NTA

CP-NTA

GP-NTA

HP-TED

CP-TED

GP-TED

≥35

≥25

≥20

≥20

≥15

≥10

≥20

≥15

≥10

Max flowrate

1500cm/h

1500cm/h

1500cm/h

Max Pressure

1MPa

1MPa

1MPa

Storage

4-30°C (20% ethanol)

4-30°C (20% ethanol)

4-30°C (20% ethanol)

Reducing agent resistance

No

β-Me≤100mM; DTT≤5mM

β-Me≤100mM; DTT≤5mM; TCEP≤5mM

Feature

High protein capacity

Low metal ion leaching rate

Resistant to 10 mM ethylenediaminetetraacetic acid   (EDTA), 0.5M NaOH, and 6M guanidine-HCl for 24 hours; resistant to 500mM imidazole, 100mM ethylenediaminetetraacetic acid (EDTA) for 2 hours

 

Bogen can provide different ligand metal chelating resin with different pore sizes according to customer needs.

 

His tag protein purification resin

 

For different purification requirements, Bogen also provides His-tag protein purification resins based on agarose soft gel matrix. Considering different His protein binding ability and transition metal ion selectivity, we also provide IMAC products without chelated metal ions, users can choose to chelate Cu2+, Zn2+, Co2+ and other transition metal ions.

 

Product Features

Product

His tag protein purification resin (HP/CP/GP-IMAC

His tag protein purification resin (IMAC

Matrix

Polyacrylate hard gel

Agarose soft gel

Ligand

NTA

NTA

Average particle size

70μm

90μm

Max flowrate

1500cm/h

500cm/h

Max Pressure

1MPa

0.3MPa

Storage

4-8(20% ethanol

4-8 (20% ethanol)

Feature

Low metal ions leaching rate, and the type of metal ions   can be freely selected

 

Case Study


Purifying His-fusion protein from supernatant of E.Coli lysate.


His-tag protein purification resin 


Ordering Information


HP series

Product Name

Part No.

Specification

Product Name

Part No.

Specification

His tag protein purification resin (HP-IDA)

BG11-1110-02

50mL

His tag protein purification resin (HP-NTA)

BG11-1111-02

50mL

BG11-1110-04

500mL

BG11-1111-04

500mL

BG11-1110-05

1L

BG11-1111-05

1L

BG11-1110-07

10L

BG11-1111-07

10L

BG11-1110-08

20L

BG11-1111-08

20L

His tag protein purification resin (HP-TED)

BG11-1113-02

50mL

His tag protein purification resin (HP-IMAC)

BG11-1101-02

50mL

BG11-1113-04

500mL

BG11-1101-04

500mL

BG11-1113-05

1L

BG11-1101-05

1L

BG11-1113-07

10L

BG11-1101-07

10L

BG11-1113-08

20L

BG11-1101-08

20L

 

CP series

Product Name

Part No.

Specification

Product Name

Part No.

Specification

His tag protein purification resin (CP-IDA)

BG11-2110-02

50mL

His tag protein purification resin (CP-NTA)

BG11-2111-02

50mL

BG11-2110-04

500mL

BG11-2111-04

500mL

BG11-2110-05

1L

BG11-2111-05

1L

BG11-2110-07

10L

BG11-2111-07

10L

BG11-2110-08

20L

BG11-2111-08

20L

His tag protein purification resin (CP-TED)

BG11-2113-02

50mL

His tag protein purification resin (CP-IMAC)

BG11-2101-02

50mL

BG11-2113-04

500mL

BG11-2101-04

500mL

BG11-2113-05

1L

BG11-2101-05

1L

BG11-2113-07

10L

BG11-2101-07

10L

BG11-2113-08

20L

BG11-2101-08

20L

 

Agarose series

Product Name

Part No.

Specification

Product Name

Part No.

Specification

His tag protein purification resin (IDA)

8G11-0010-02

50mL

His tag protein purification resin (NTA)

BG11-0011-02

50mL

BG11-0010-04

500mL

BG11-0011-04

500mL

BG11-0010-05

1L

BG11-0011-05

1L

BG11-0010-07

10L

BG11-0011-07

10L

BG11-0010-08

20L

BG11-0011-08

20L

His tag protein purification resin (TED)

BG11-0013-02

50mL

His tag protein purification resin (IMAC)

BG11-0001-02

50mL

BG11-0013-04

500mL

BG11-0001-04

500mL

BG11-0013-05

1L

BG11-0001-05

1L

BG11-0013-07

10L

BG11-0001-07

10L

BG11-0013-08

20L

BG11-0001-08

20L

 

His tag protein purification prepacked column

Product Name

Specification

Applicable Molecular Weight

Capacity

Max Pressure

Max Flow rate

Application/Feature

His tag protein purification column (HP-IDA)

5mL

10mL

≤200KD

35mg/mL

1MPa

1000cm/h

For His tag protein purification, high dynamic capacity, high maximum pressure

His tag protein purification column (HP-NTA)

5mL

10mL

≤200KD

20mg/mL

1MPa

1000cm/h

For His tag protein purification, low ligand leaching rate, high maximum pressure

His tag protein purification column (HP-TED)

5mL

10mL

≤200KD

≥20mg/mL

1MPa

1000cm/h

For purification of His-tagged proteins expressed in eukaryotic cells, resistant to a certain concentration of EDTA, directly cleaned with NaOH,   high maximum pressure

His tag protein purification column (GP-IDA)

5mL

10mL

≥150KD

20mg/mL

1MPa

1000cm/h

For purification of His-tagged proteins with extremely large molecular weight, or to capture His-tagged proteins from relatively viscous liquids.

His tag protein purification column (GP-NTA)

5mL

10mL

≥150KD

10mg/mL

1MPa

1000cm/h

For purification of His-tagged proteins with extremely large molecular weight, or to capture His-tagged proteins from relatively viscous liquids, low ligand   leaching rate, high maximum pressure

His tag protein purification column (GP-TED)

5mL

10mL

≥150KD

10mg/mL

1MPa

1000cm/h

Purification of ultra-large molecular weight His-tagged proteins expressed in eukaryotic cells, withstands a certain concentration of EDTA,   can be directly cleaned with NaOH, and has high maximum pressure

His tag protein purification column (IDA)

5mL

10mL

≤200KD

40mg/mL

0.3MPa

600cm/h

For purification of His tag protein, high dynamic capacity

His tag protein purification column (NTA)

5mL

10mL

≤200KD

20mg/mL

0.3MPa

600cm/h

For purification of His tag protein, low ligand leaching

His tag protein purification column (TED)

5mL

10mL

≤200KD

15mg/mL

0.3MPa

600cm/h

For purification of His-tagged proteins expressed in eukaryotic cells. It can withstand a certain concentration of EDTA and can be directly cleaned   with NaOH.

 

 

 


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