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Duoning cell culture flasks are manufactured with excellent and rigorous process, ensures the smooth body of the flasks, and reduces the risk of foaming during culture.

Duoning cell culture flasks have multiple uses, including cultivate mammalian cells (CHO cells, HEK 293 cells) for recombinant protein production. Excellent product design can greatly improve the oxygen transfer efficiency inside the flask itself , thereby providing better growth environment for cells.

Duoning cell culture flasks can be used with other cell culture equipment and consumables produced by Duoning, including carbon dioxide incubators with shakers, bioreactors.

Products Benefits

? Meet regulations of USP6

? Blow-injection molding, avoiding surface scars

? Excellent chemical resistance, can withstand a variety of chemicals

? Good transparency, clear scale, easy to observe

? Flask neck sizes conforms to the international SBS standard, suitable for quick connection of bioreactors

Features Of 2.8 L & 5 L Cell Culture Flasks

? Equipped with a 0.2 μm air-permeable cap, which can better mix the cells and the culture media, increase the air permeability, and enable the cells to grow to higher density.

? The handle design of the 5 L culture flask is easy to pick up, avoiding the risk of contamination caused by the customer's hand being too close to the flask neck during use.

? The bottom of the 2.8 L culture bottle is optimized, which saves space, greatly improves the utilization rate of the incubator, and reduces customer R&D and production costs.

? Duoning cell culture flask adopts extrusion blowing technology, the flask body is smooth, which reduces the risk of cell adhesion and media foaming.

? Duoning cell culture flasks are sterilized by cobalt 60 radiation, and the aseptic independent packaging is convenient for use.

Cell Culture Flask

Aseptic transfer cap

Duoning aseptic transfer caps are used for aseptic transfer during the expansion of suspension cells, effectively reducing the risk of contamination. The aseptic transfer cap consists of two ports: The vent port is connected to a 0.2 um air filter; The inner end of the liquid delivery port is connected to a chemically resistant hard tube immersed in the bottom part of the flask, and the outer side is connected to a 1/8”-1/4” thermoplastic tube.

Cell Culture Flask

Product benefits

? Compatible with Duoning 2.8 L & 5 L culture flasks;

? Double-layer independent aseptic packaging;

? Disposable, ready-to-use.

Ordering information

Cell Culture Flask

Part No.	Volume	Type	Height	Neck Diameter	Bottom Diameter	Sterilization	Pcs/Pack	Pcs/Box
DNFL02010125	125 mL	PETG	113.5 mm	34 mm	66 mm	Yes	1	24
DNFL02010250	250 mL		138.2 mm	34 mm	83 mm			12
DNFL02010500	500 mL		177.5 mm	39 mm	101 mm			12
DNFL02011000	1,000 mL		211.5 mm	39 mm	127 mm			6
DNFL02032800	2.8 L	K-Resin	243.3 mm	96.2 mm	164 mm			6
03-5000	5 L, 4 pcs	K-Resin	253.2 mm	96.2 mm	228 mm			4
DNFL02020125	125 mL	PC	113.5 mm	34 mm	66 mm			24
DNFL02020250	250 mL		138.2 mm	34 mm	83 mm			12
DNFL02020500	500 mL		177.5 mm	39 mm	101 mm			12
DNFL02021000	1,000 mL		211.5 mm	39 mm	127 mm			6

Aseptic Transfer Cap

Part No.	Sterilization	Pcs/pack	Pcs/box
DNTC020106P	Yes	1	10

Culture Parameter Recommendation

Cell type	Size	Recommended max capacity (mL)	Max speed (rpm)	Shaker track specification (mm)
CHO-S	125 mL	30	130	25
	250 mL	60	130	25
	500 mL	150	130	25
	1,000 mL	350	130	25
	2,800 mL	800	90	25
	5,000 mL	2,500	90	25
HEK	125 mL	30	130	15
SF9	125 mL	30	130	15
	500 mL	200	150	31.75
	1,000 mL	300	120-150	31.75
	2,800 mL	1,000	100-150	31.75
	5,000 mL	3,500	90-100	31.75

Performance Data

CHOK1 cell growth results after 10x passages

Cell Culture Flask

Volume	Optimal working volume	Effective volume	Optimal speed	Suggested speed range
125 mL	25 – 30 mL	8 - 35 mL	110	90 - 110
250 mL	60 mL	36 – 100 mL
500 mL	100 mL	50 – 200 mL
1,000 mL	200 mL	101 – 400 mL
2.8 L	1.4 L	0.9 – 1.6 L
5 L	2 L	1.7 – 3.2 L	90
Note：Duoning culture flask specification only

Cell Culture Flask & Aseptic Transfer Cap