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ELISA Kit
Home > Technology > Reagent > ELISA Kit > Intelli Nuclease ELISA Kit

Intelli Nuclease ELISA Kit


The Intelli nuclease ELISA kit is used to quantitatively detect the content of omnipotent nuclease residues in samples. It can be used for Duoning Intelli nuclease, and for omnipotent nuclease of other brands.

 

Intelli Nuclease ELISA Kit

Specifications

Detection Method

Detection Type

Detection Scope

Colorimetry (one-step method)

Sandwich ELISA (quantitative)

0.2 ng/mL-20 ng/mL




Sensitivity

Estimated duration

Storage condition

0.06 ng/mL

2 hours and 15 minutes

2-8

 

Detection principle


The Intelli nuclease ELISA kit adopts double-antibody sandwich principle, and the anti-omnipotent nuclease antibody is coated on the ELISA plate, the sample to be tested is added and washed, and then the antibody labeled with horseradish peroxidase (HRP) is added for incubation to form an antibody-antigen-enzyme-labeled antibody complex, after thorough washing, the substrate TMB is added for color development, and the substrate TMB is transformed from colorless to blue under the catalysis of peroxidase, and is terminated by acid solution, finally forming a yellow clear liquid. There is a positive correlation between the color intensity and the content of omnipotent nuclease in the sample to be tested.

 

Standard curve

Intelli Nuclease ELISA Kit

 

Linear Range & Recovery

 

Theoretical conc. ng/mL

OD

Calibrated OD

Measured value

ng/mL

Linear correlation coefficient (R2)

Recovery

Test result

20.00

2.767

2.754

2.725

19.53

1.0000

97.6%

Pass

1.00

0.324

0.325

0.289

0.97

96.8%

0.50

0.184

0.180

0.146

0.50

99.3%

0.33

0.132

0.128

0.094

0.33

100.0%

0.25

0.106

0.105

0.070

0.25

99.7%

0.20

0.088

0.085

0.051

0.18

91.5%

 

LoD

Detection times

OD

Calibrated OD

Mn

SD

Mn+2SD

LoD ng/mL

Test result

1

0.029

-0.007

-0.0067

0.0007

-0.0053

<0

Pass

2

0.029

-0.007

3

0.029

-0.007

4

0.029

-0.007

5

0.029

-0.007

6

0.031

-0.005

7

0.029

-0.007

8

0.029

-0.007

 

LLoD

Detection times

OD

Calibrated OD

Measured value ng/mL

Mn

SD

CV

Test result

1

0.090

0.054

0.19

0.1976

0.0039

2.0%

Pass

2

0.092

0.056

0.20

3

0.090

0.054

0.19

4

0.091

0.055

0.20

5

0.093

0.057

0.20

6

0.092

0.056

0.20

7

0.091

0.055

0.20

8

0.090

0.054

0.19

 

Compatibility test with omnipotent nuclease of other brands

Competitor

Brand H

Brand S

Brand Y 1

Brand Y2

Brand B

Compatibility

 ***

***

**

***

***

Note: Three stars is a perfect fit, and so on

 

Precision Test

Sample (ng/mL): 0.75

Reagent lot #

Test times

OD

Calibrated OD

Determination value ng/mL

Intra-run CV

Inter-run CV

Test result

Lot 1

1

0.248

0.212

0.71

3.2%

3.0%

Pass

2

0.258

0.222

0.74

3

0.249

0.213

0.71

4

0.255

0.219

0.73

5

0.245

0.209

0.70

6

0.264

0.228

0.76

7

0.246

0.210

0.70

8

0.246

0.210

0.70

Lot 2

1

0.270

0.241

0.74

1.9%

2

0.267

0.238

0.73

3

0.268

0.239

0.73

4

0.264

0.235

0.72

5

0.268

0.239

0.73

6

0.269

0.240

0.74

7

0.273

0.244

0.75

8

0.278

0.249

0.77

Lot3

1

0.305

0.278

0.73

2.0%

2

0.316

0.289

0.76

3

0.318

0.291

0.76

4

0.316

0.289

0.76

5

0.323

0.296

0.78

6

0.315

0.288

0.76

7

0.310

0.283

0.74

8

0.311

0.284

0.75

 

Sample (ng/mL): 3

Reagen lot #

Test times

OD

Calibrated OD

Determination value ng/mL

Intra-run CV

Inter-run CV

Test result

Lot 1

1

0.797

0.761

2.75

4.2%

3.9%

Pass

2

0.794

0.758

2.74

3

0.872

0.836

3.08

4

0.857

0.821

3.01

5

0.839

0.803

2.93

6

0.862

0.826

3.03

7

0.830

0.794

2.89

8

0.833

0.797

2.91

Lot 2

1

0.867

0.838

2.81

1.8%

2

0.875

0.846

2.84

3

0.862

0.833

2.79

4

0.860

0.831

2.78

5

0.889

0.860

2.90

6

0.874

0.845

2.84

7

0.877

0.848

2.85

8

0.898

0.869

2.94

Lot 3

1

1.039

1.012

3.03

2.7%

2

1.007

0.980

2.92

3

1.070

1.043

3.15

4

1.034

1.007

3.01

5

1.022

0.995

2.97

6

1.066

1.039

3.13

7

1.038

1.011

3.03

8

1.016

0.989

2.95

 

Sample (ng/mL):    12

Reagent lot #

Test times

OD

Calibrated OD

Determination value ng/mL

Intra-run CV

Inter-run CV

Test result

Lot 1

1

2.145

2.109

11.10

4.6%

 7.2%

Pass

2

2.256

2.220

12.21

3

2.211

2.175

11.74

4

2.217

2.181

11.80

5

2.224

2.188

11.88

6

2.294

2.258

12.62

7

2.128

2.092

10.94

8

2.214

2.178

11.77

Lot 2

1

2.284

2.255

11.02

2.0%

2

2.321

2.292

11.35

3

2.331

2.302

11.44

4

2.331

2.302

11.44

5

2.352

2.323

11.63

6

2.314

2.285

11.28

7

2.305

2.276

11.21

8

2.277

2.248

10.96

Lot 3

1

2.528

2.501

11.67

5.9%

2

2.639

2.612

12.78

3

2.598

2.571

12.36

4

2.742

2.715

13.90

5

2.589

2.562

12.27

6

2.715

2.688

13.60

7

2.695

2.668

13.38

8

2.674

2.647

13.15

 

 

Ordering information

 

Product Name

Specification

Part No.

Intelli Nuclease ELISA Kit

96 wells/kit

DNRG030301

 



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  • Tel.: +86 021 6434 0155
  • E-Mail: marketing@duoningbio.com
  • Address: Building 30, No. 1525 Minqiang Road, Songjiang District, Shanghai
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