日韩成人一区二区三区在线观看,亚洲爆乳成av人在线视菜奈实,一级特黄aaa大片在线观看成人一级片在线观看,欧美一级a视频在线观看,久久综合狠狠色综合伊人,国产亂倫近親相姦视频,日韩免费无码视频一区二区三区,97久久精品亚洲中文字幕无码,久爱无码精品免费视频,超碰97人人模人人爽人人

Hydroxyapatite Chromatography Resin
Home > Technology > Chromatography Resin > Hydroxyapatite Chromatography Resin > Hydroxyapatite Chromatography Resin
  • Hydroxyapatite Chromatography Resin
  • Hydroxyapatite Chromatography Resin

Hydroxyapatite Chromatography Resin

 

Hydroxyapatite is a biological material composed of calcium and phosphate. It exhibits excellent biocompatibility and biological activity. Notably, chromatography resins made from hydroxyapatite demonstrate high separation efficiency, selectivity, reproducibility, and stability. As a result, hydroxyapatite has gained widespread recognition in the field of biomolecule separation and purification.

 

Principles of Separation and Purification

 

Hydroxyapatite chromatography resin operates on a unique principle of separation and purification that combines both cation exchange and metal chelation. The phosphate ions within its crystal structure can bind to positively charged proteins, demonstrating cation exchange properties, which can be eluted by varying the NaCl concentration. Meanwhile, calcium ions interact with free carboxyl groups on proteins through metal chelation, and these can be eluted by adjusting the phosphate concentration. Consequently, the chromatography resin can be eluted using a single gradient of sodium phosphate or through a dual gradient elution model. In the latter approach, elution begins with a NaCl gradient, followed by equilibration with a low-concentration sodium phosphate buffer, and concludes with elution using a sodium phosphate concentration gradient. This method enhances the efficiency of impurity removal.

 

Hydroxyapatite Chromatography Resin Properties

 

Hydroxyapatite is classified into two types based on distinct ceramicization processes: Type I and Type II.

 

? Type I has a small pore size, which allows for greater retention of proteins and a higher dynamic binding capacity for acidic proteins. It primarily purifies most proteins effectively.

? Type II features a large pore size that allows for greater retention of macromolecules, such as antibodies and nucleic acids. Additionally, it possesses a higher dynamic capacity, making it more suitable for the purification of antibodies and nucleic acids.

 

Type

                                                                                 Type I

                                                                                Type II

Average pore size

30 nm

40 nm

Application

Purification of most proteins (<100 kD)

Antibodies & nucleic acids purification

pH range

6.5 - 14

Max working pressure

9 MPa

 

                                              Hydroxyapatite Chromatography Resin

 

Advantages of Hydroxyapatite Chromatography Resin:

 

? High dynamic capacity, high flow rate, and high productivity;

? Enhanced chemical stability and mechanical strength provide a longer lifespan;

? The rigid structure ensures stability at a pH greater than 6.5 and can be cleaned with sodium hydroxide (NaOH);

? Good batch-to-batch reproducibility and ease of scalability.

 

You can freely choose between cationic and metal chelation methods to separate and purify proteins or other molecules.

 

Applications of Hydroxyapatite Chromatography Resin

 

Monoclonal Antibody Purification

 

Hydroxyapatite chromatography resin is utilized for the purification of monoclonal antibodies. This process involves capturing the target antibody through a phosphate gradient and eluting it using a non-phosphate system, such as a sodium chloride gradient. This method effectively removes antibody aggregates, leached Protein A ligands, endotoxins, and other impurities, thereby ensuring the purity and yield of the final product.

 

Nucleic Acid Purification

 

Hydroxyapatite chromatography resin can interact with the phosphate group on nucleic acids through the C site and can be directly used to purify mRNA. Unlike traditional anion exchange chromatography resins, the retention time of mRNA on hydroxyapatite chromatography resin increases with the number of mRNA bases, providing better resolution for nucleic acids of varying lengths.

 

Polysaccharide Vaccine Purification

 

The impurities in the production of polysaccharide vaccines primarily consist of host cell proteins (HCP), host cell DNA, pigments, and other polysaccharide substances. Hydroxyapatite chromatography resin can specifically absorb HCP and DNA, thereby enhancing the purity and effectiveness of polysaccharide vaccines.

 

Now, Bogen, a Duoning Company, has launched our new generation of hydroxyapatite chromatography resin, including both Type I and Type II, to better support your chromatography process efforts.

 

Hydroxyapatite Chromatography Resin

 

Product name

Product number

Specification

Hydroxyapatite chromatography resin (Type I)

BG81-1I10-02

50 mL

BG81-1I10-04

500 mL

BG81-1I10-05

1 L

BG81-1I10-07

10 L

BG81-1I10-08

20 L

Hydroxyapatite chromatography resin (Type II)

BG82-1I10-02

50 mL

BG82-1I10-04

500 mL

BG82-1I10-05

1 L

BG82-1I10-07

10 L

BG82-1I10-08

20 L





Contact Us
  • Tel.: +86 021 6434 0155
  • E-Mail: marketing@duoningbio.com
  • Address: Building 30, No. 1525 Minqiang Road, Songjiang District, Shanghai
Message

Copyright ? Shanghai Duoning Biotechnology Co., Ltd. All Rights Reserved Sitemap | Technical Support: Reanod

WeChat
WeChat

Message-

Duoning Biotechnology

+86 021 6434 0155

久久久国产精品亚洲一区| 欧美黑人粗暴多交高潮水最多| 狠狠做深爱婷婷久久综合一区| 无码专区AAAAAA免费视频| 免费一级做a爰片久久毛片潮| 久久精品视频免费播放| 国产精品美女久久久久| 亚洲AV无一区二区三区久久| 日本乱人伦中文字幕| 秋霞2016午夜限制电影在线| 国产精品毛片a∨一区二区三区| 久久上司侵犯部下人妻| 久久精品人妻中文视频| 四虎一区二区成人免费影院网址| 无码视少妇视频一区二区三区| 亚洲女久久久噜噜噜熟女| 精品无码午夜福利理论片| 91福利久久AⅤ无码精品色午麻豆精品国产| 妺妺窝人体色www在线观看| 女人18毛片a级毛片一区**区| 人妻熟妇久久久久久XXX| 播放国产精品亚洲av综合成久久久久久三级| 最近2024中文字幕免费直播| 奔跑吧蔷薇日本免费一区香蕉视频| 久久久无码精品亚洲日韩| 手机毛片在线观看| 国产99视频在线观看| 久久综合亚洲鲁鲁九月天| 少妇人妻无码一区二区三区三级| 欧美人与动性xxxxx杂性| 久久久久无码国产精品不卡| 亚洲乱码一区二区三区在线欧美| 国产高清一区二区三区直播| 亚洲欧美一区二区三区久久| 亚洲精品欧美精品日韩精品| 免费午夜伦费影视在线观看| 少妇无码自慰毛片久久久久| 日本熟妇色XXXXX日本免费看| 精品久久久久中文字幕日本| 午夜精品一区二区三区在线观看| 92国产精品午夜福利无毒不卡|