日韩成人一区二区三区在线观看,亚洲爆乳成av人在线视菜奈实,一级特黄aaa大片在线观看成人一级片在线观看,欧美一级a视频在线观看,久久综合狠狠色综合伊人,国产亂倫近親相姦视频,日韩免费无码视频一区二区三区,97久久精品亚洲中文字幕无码,久爱无码精品免费视频,超碰97人人模人人爽人人

Intelli Nuclease
Home > Technology > Reagent > Intelli Nuclease > Intelli Nuclease

Intelli Nuclease


Biological products produced in cells, such as viral vaccines and viral vectors, contain residual DNA from the host cell substrates. Theoretically, residual DNA could deliver activated oncogenes and potentially infectious viral genomes to patients and induce carcinogenic or infectious events. Regulatory agencies around the world have set regulatory limits for residual DNA to ensure patient safety. During the production process, manufacturers need to reduce the content of such impurities through specific methods to meet the regulatory requirements. In addition, nucleic acid substances produced during upstream cell culture or cell lysis can significantly increase the viscosity of the solution, affecting the efficiency of downstream process steps.

 

Intelli nuclease is an  omnipotent endonuclease expressed in recombinant E. coli, originally from Serratia marcescens. The enzyme is composed of two identical subunits (approximately 27.5KDa), is a non-specific nuclease that can completely and efficiently degrade any form of DNA and RNA (single-stranded, double-stranded, linear, circular, supercoiled) into oligonucleotide of 2-5 bases in length.

 

Applications

?   Remove DNA/RNA in protein purification process, to reduce viscosity, simplify clarification process, and effectively improve yields

?   Prepare samples for ELISA, chromatography, 2D electrophoresis, and blotting, improving resolution and recovery. Eliminate host cell DNA/RNA residues during vaccine and protein manufacturing, improving product safety.

?   Prevent cell clumping during cell culture, including PBMC, chimeric cell mixture preparation.

 

Reaction Conditions

?   Optimum reaction conditions: pH 6-10 (prefer pH 8-9), 1-2 mM Mg2+, 0-150 mM NaCI, 0-42°C (prefer 37 °C)

?   Enzyme activity will be significantly inhibited (reduction rate>50%) if monovalent cations >300 mM, PO43+>100 mM, (NH4)2SO4>100 mM

?   The enzyme is compatible with PMSF (1 mM), EDTA (1 mM), reducing agents (e.g. DTT) , detergents (e.g. Triton).

 

Manufacturing standard

Product Name

Nuclease, Serratia marcescens extracellular endonuclease

CAS No.

9025-65-4

Form

Active substance; monomer

Appearance

Colorless, no particles

Expression system

Non-pathogenic Escherichia Coli, BL21(DE3)

Source

Recombinant E. coli modified by nuclease gene

Formula

50 mM Tris-HCl, 100 mM NaCl, 5% glycerol, 2 mM Mg2+, pH8.0

Storage

-20 ℃, avoid repeated frozen-thaw

Endotoxin content

< 0.5 EU/mg

SEC-HPLC (%)

≥99%

SDS-PAGE (%)

≥99%

Molecular weight

27501.51 Da

Theoretical molecular weight

27500.81 Da

Enzyme activity

>250 U/μL


 Stability Data


Intelli Nuclease

?   0.4%(w/v) of TritonTM X-100 has no effect on enzyme activity, and 1.0%(w/v) of TritonTM X-100 hads inhibitory effect on enzyme activity.

?   PMSF, maleic acid and 2-mercaptoethanol have no effect on enzyme activity at 1 mM

?   EDTA-2Na inhibited about 60% of the enzyme activity at 1 mM concentration.

 

Reaction condition

Relative enzyme activity

Reaction condition

Relative enzyme activity

Reaction condition

Relative enzyme activity

Triton-0.4% (W/V)

>80%

PMSF-0mM

>80%

Control

100%

Triton-1.0% (W/V)

>20%

PMSF-1mM

>80%

EDTA-2Na-1mM

>60%

Maleic acid-1mM

>80%

PMSF-3mM

>80%

2-Mercaptoethanol

>80%

 

Ordering Information

Specifications

Part No.

500 KU/vial

DNRG0302500-GMP (in-stock)

1 MU/vial

DNRG0302001-GMP (in-stock)

5 MU/vial

DNRG0302005-GMP (in-stock

 





Relevant Documents

Contact Us
  • Tel.: +86 021 6434 0155
  • E-Mail: marketing@duoningbio.com
  • Address: Building 30, No. 1525 Minqiang Road, Songjiang District, Shanghai
Message

Copyright ? Shanghai Duoning Biotechnology Co., Ltd. All Rights Reserved Sitemap | Technical Support: Reanod

WeChat
WeChat

Message-

Duoning Biotechnology

+86 021 6434 0155

亚洲伦理一区二区| 亚洲风情亚aⅴ在线发布| 丁香综合五月天亚洲日韩欧美| 亚洲日韩国产AV无码无码精品| 日本XXXX色视频在线观看免费不卡| 人妻免费久久久久久久了| 欧美日韩视频在线观看一区| 天堂av无码av一区二区三区| 欧美内射深插日本少妇| 精品21国产成人综合网在线| 日本一区二区三区不卡免费| 亚洲AV日韩AV无码AV| 久久99国产一区二区三区| 国产精品乱码高清在线观看| 国产乱人伦精品一区二区在线观看| 日本乱人伦中文字幕| 无码成人动漫一区二区三区| 亚洲中文久久久久久精品国产| 一卡二卡亚洲乱码一卡二卡| 国产欧美亚洲精品a| 99国产精品久久99| 乱色熟女综合一区二区三区| 国产小受呻吟GV视频在线观看| 精品国产AV无码一区二区三区| 午夜精品久久久久久毛片| 久久夜噜噜噜亚洲AV蜜臀| 竹菊影视欧美日韩一区二区三区| 成熟人妻av无码专区| 办公室双腿打开揉弄在线观看| 日韩内射激情视频在线播放免费| 色狠狠色噜噜Av天堂一区| 欧美一级一区二区中文字幕| 亚洲AⅤ综合在线欧美一区| 成人性色生活片免费看成人性| 日产无人区一线二线三线观看| 亚洲日韩成人片在线播放| 国产欧美日韩精品一区二区三区蜜桃不卡| 夜夜骚AV-D,亚洲VA中文字幕无码毛片| 激情久久AV一区AV二区AV三区| 久久婷婷秘精品果冻传媒| 中文字幕一区2区,在线日韩中文字幕|