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Ion Exchange Chromatography Resin
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Ion exchange resin

 

Ion exchange resin is one of the most used protein purification methods, which can separate target protein from impurities based on the difference in the isoelectric point of the proteins.

 

The hard gel chromatography resin developed by Bogen (A Duoning company) uses high-strength hydrophilic polyacrylate beads as matrix, and unique flexible hydrophilic modification technology to further eliminate the non-specific binding caused by the strong hydrophobic micro-surface of chromatography resins based on hard gel, which increases the recovery of active protein, improves the robustness and repeatability of process.

 

Bogen provides wide range of particle sizes for different purification process stages: for capture or crude feed purification, we recommend chromatography resin products with average particle size of 70 μm or 120 μm, for high-viscosity feed, consider resins with particle size of 120 μm or 200 μm; for polishing step, we recommend resins with average particle size of 40 μm or 70 μm. And please contact Bogen for more supports, if you need other chromatography resin products with non-standard particle sizes.

 

?   Max pressure up to 10 bar

?   Liner flowrate up to 1000 cm/h

?   Loading capacity 60-120mg/ml (depends on application)

 

Function Groups

DEAE, Q, CM, S

 

Product Series

?   HP series: use for purification of peptide and recombinant proteins

?   CP series: use for purification of recombinant proteins and antibodies

?   GP series: use for purification of antigens of vaccine and virus-based products

 

Standard particle size: 40 μm, 70 μm, 120 μm

 

Ion exchange resinProduct Specification

 

Series

Applicable Molecular Weight Range

Mode

Particle Size

Max Flow Rate

Dynamic Binding Capacity (mg/mL)

HP

≤200KD

DEAE

40 μm

70 μm

1500cm/h

90-100 (BSA)

Q

100-110 (BSA)

CM

45-50 (LZM)

S

55-60 (LZM)

CP

100KD-300KD

DEAE

40 μm

70 μm

1500cm/h

80-85 (BSA)

Q

95-105 (BSA)

CM

45-50 (LZM)

S

50-55 (LZM)

GP

≥150KD

DEAE

40 μm

70 μm

1500cm/h

50-60 (BSA)

Q

80-90 (BSA)

CM

35-40 (LZM)

S

25-30 (LZM)

 

Ion exchange resinProduct Features

Bogen uses a series of patented technologies to optimize the particle size, pore structure and micro-surface physical and chemical properties of polymer hard gel, achieving higher basic resolution for resin itself. Comparing to agarose resins, it provides higher resolution under same process conditions.

 

Ion exchange resin

Strong cation exchange media resolution

 

Ion exchange resins: A: Bogen HPS-60; B: Product-S; C: Product-S ImpAct

Column size: 1.6cm ID x 2.5cm

Flowrate: 2mL/min

Loading volume: 5mL

Sampe: 1. Cytochrome C (1mg/mL); 2. Lysozyme (1mg/mL)

 

The strong cation exchange resin from Bogen can provide higher resolution under the same conditions.

 

Besides high resolution, the chromatography resin from Bogen provides ideal pressure-flowrate curve, which helps to maintain low back pressure during scale-up. Under increased flowrate or column height, the pressure change is relatively smooth, and much lower than agarose-based resin, which is desired for scale-up production processes.

 

 

Ion exchange resin

Pressure vs. flowrate for HPS strong cation exchange resin

 

Bogen uses multi-scale micro-nano structure control technology to produce chromatography resin with high specific surface area porous structure. We designed resins with optimum pore size and arm space through computer simulation technology and analyzing hydraulic size of different types of biomacromolecules, achieving high dynamic binding capacity and adsorption and desorption in fast mass transfer.

 

Ion exchange resin

Pressure vs. flow rate curves of ion exchange resins with different pore sizes

 

Super macro-porous resin for purification of vaccine/virus

 

Vaccine/virus purification is featured application of super macroporous ion-exchange resin developed by Bogen. Purifying vaccine antigen/virus vector using GPQ strong anion exchange resin or GPS strong cation exchange resin provides advantages of high loading (1-30 CVs), high purity and high yield.

 

Applications:

?   Virus vaccine purification

?   Virus-like particles (VLP) purification

?   Virus vector purification, including LV、AV、 AAV

 

Ion exchange resin

Purification of vaccine antigen

 

Case study

 

Ion exchange resin

Chromatogram of model vaccine antigen purification

 

Resin: GPQ60 strong anion exchange resin

Column size: 1.6cm ID × 15cm

Flowrate: 5mL/min

Sample: A model vaccine antigen

Purity: Impurity removal rate >90%

Recovery rate: >80%

 

Ion exchange resinOrdering information

 

Anion Exchange Resins – HP Series

Product name

Part No.

Specification

Product name

Part No.

Specification

HPDEAE-60 weak anion exchange resin

BG31-1100-02

50mL

HPDEAE-30 weak anion exchange resin

BG31-1200-02

50mL

BG31-1100-04

500mL

BG31-1200-04

500mL

BG31-1100-05

1L

BG31-1200-05

1L

BG31-1100-07

10L

BG31-1200-07

10L

BG31-1100-08

20L

BG31-1200-08

20L

HPDEAEHL-60 weak anion exchange resin

BG31-1120-02

50mL

HPDEAEHL-30 weak anion exchange resin

BG31-1220-02

50mL

BG31-1120-04

500mL

BG31-1220-04

500mL

BG31-1120-05

1L

BG31-1220-05

1L

BG31-1120-07

10L

BG31-1220-07

10L

BG31-1120-08

20L

BG31-1220-08

20L

HPQ-60 strong anion exchange resin

BG32-1110-02

50mL

HPQ-30 strong anion exchange resin

BG32-1210-02

50mL

BG32-1110-04

500mL

BG32-1210-04

500mL

BG32-1110-05

IL

BG32-1210-05

1L

BG32-1110-07

10L

BG32-1210-07

10L

BG32-1110-08

20L

BG32-1210-08

20L

HPQHL-60 strong anion exchange resin

BG32-1120-02

50mL

HPQHL-30 strong anion exchange resin

BG32-1220-02

50mL

BG32-1120-04

500mL

BG32-1220-04

500mL

BG32-1120-05

IL

BG32-1220-05

1L

BG32-1120-07

10L

BG32-1220-07

10L

BG32-1120-08

20L

BG32-1220-08

20L

 

Anion Exchange Resins – CP series

Product name

Part No.

Specification

Product name

Part No.

Specification

CPDEAE-60 weak anion exchange resin

BG31-2100-02

50mL

CPDEAE-30 weak anion exchange resin

BG31-2200-02

50mL

BG31-2100-04

500mL

BG31-2200-04

500mL

BG31-2100-05

1L

BG31-2200-05

1L

BG31-2100-07

10L

BG31-2200-07

10L

BG31-2100-08

20L

BG31-2200-08

20L

CPDEAEHL-60 weak anion exchange resin

BG31-2120-02

50mL

CPDEAEHL-30 weak anion exchange resin

BG31-2220-02

50ml

BG31-2120-04

500mL

BG31-2220-04

500mL

BG31-2120-05

1L

BG31-2220-05

1L

BG31-2120-07

10L

BG31-2220-07

10L

BG31-2120-08

20L

BG31-2220-08

20L

CPQ-60 strong anion exchange resin

BG32-2110-02

50mL

CPQ-30 strong anion exchange resin

BG32-2210-02

50mL

BG32-2110-04

500mL

BG32-2210-04

500mL

BG32-2110-05

1L

BG32-2210-05

1L

BG32-2110-07

10L

BG32-2210-07

10L

BG32-2110-08

20L

BG32-2210-08

20L

CPQHL-60

strong anion exchange resin

BG32-2120-02

50mL

CPQHL-30

strong anion exchange resin

BG32-2220-02

50mL

BG32-2120-04

500mL

BG32-2220-04

500mL

BG32-2120-05

1L

BG32-2220-05

1L

BG32-2120-07

10L

BG32-2220-07

10L

BG32-2120-08

20L

BG32-2220-08

20L

 

Anion Exchange Resins – GP series

Product name

Part No.

Specification

Product name

Part No.

Specification

GPDEAE-60 weak anion exchange resin

BG31-3100-02

50mL

GPDEAE-30 weak anion exchange resin

BG31-3200-02

50mL

BG31-3100-04

500mL

BG31-3200-04

500mL

BG31-3100-05

1L

BG31-3200-05

1L

BG31-3100-07

10L

BG31-3200-07

10L

BG31-3100-08

20L

BG31-3200-08

20L

GPDEAEHL-60 weak anion exchange resin

BG31-3120-02

50mL

GPDEAEHL-30 weak anion exchange resin

BG31-3220-02

50mL

BG31-3120-04

500mL

BG31-3220-04

500mL

BG31-3120-05

1L

BG31-3220-05

1L

BG31-3120-07

10L

BG31-3220-07

10L

BG31-3120-08

20L

BG31-3220-08

20L

GPQ-60 strong anion exchange resin

BG32-3110-02

50mL

GPQ-30 strong anion exchange resin

BG32-3210-02

50mL

BG32-3110-04

500mL

BG32-3210-04

500mL

BG32-3110-05

1L

BG32-3210-05

1L

BG32-3110-07

10L

BG32-3210-07

10L

BG32-3110-08

20L

BG32-3210-08

20L

GPQHL-60 strong anion exchange resin

BG32-3120-02

50mL

GPQHL-30 strong anion exchange resin

BG32-3220-02

50mL

BG32-3120-04

500mL

BG32-3220-04

500mL

BG32-3120-05

1L

BG32-3220-05

1L

BG32-3120-07

10L

BG32-3220-07

I0L

BG32-3120-08

20L

BG32-3220-08

20L

 

Cation Exchange Resins – HP series

Product name

Part No.

Specification

Product name

Part No.

Specification

HPCM-60 weak cation exchange resin

BG33-1110-02

50mL

HPCM-30 weak cation exchange resin

BG33-1210-02

50mL

BG33-1110-04

500mL

BG33-1210-04

500mL

BG33-1110-05

1L

BG33-1210-05

1L

BG33-1110-07

10L

BG33-1210-07

10L

BG33-1110-08

20L

BG33-1210-08

20L

HPS-60 strong cation exchange resin

BG34-1110-02

50mL

HPS-30 strong cation exchange resin

BG34-1210-02

50mL

BG34-1110-04

500mL

BG34-1210-04

500mL

BG34-1110-05

1L

BG34-1210-05

1L

BG34-1110-07

10L

BG34-1210-07

10L

BG34-1110-08

20L

BG34-1210-08

20L

 

Cation Exchange Resins – CP series

Product name

Part No.

Specification

Product name

Part No.

Specification

CPCM-60 weak cation exchange resin

BG33-2110-02

50mL

CPCM-30 weak cation exchange resin

BG33-2210-02

50mL

BG33-2110-04

500mL

BG33-2210-04

500mL

BG33-2110-05

1L

BG33-2210-05

1L

BG33-2110-07

10L

BG33-2210-07

10L

BG33-2110-08

20L

BG33-2210-08

20L

CPS-60 strong cation exchange resin

BG34-2110-02

50mL

CPS-30 strong cation exchange resin

BG34-2210-02

50mL

BG34-2110-04

500mL

BG34-2210-04

500mL

BG34-2110-05

1L

BG34-2210-05

1L

BG34-2110-07

10L

BG34-2210-07

10L

BG34-2110-08

20L

BG34-2210-08

20L

 

Cation Exchange Resins – GP Series

Product name

Part No.

Specification

Product name

Part No.

Specification

GPCM-60 weak cation exchange resin

BG33-3110-02

50mL

GPCM-30 weak cation exchange resin

BG33-3210-02

50mL

BG33-3110-04

500mL

BG33-3210-04

500mL

BG33-3110-05

1L

BG33-3210-05

1L

BG33-3110-07

10L

BG33-3210-07

10L

BG33-3110-08

20L

BG33-3210-08

20L

GPS-60 strong cation exchange resin

BG34-3110-02

50mL

GPS-30 strong cation exchange resin

BG34-3210-02

50mL

BG34-3110-04

500mL

BG34-3210-04

500mL

BG34-3110-05

1L

BG34-3210-05

1L

BG34-3110-07

10L

BG34-3210-07

10L

BG34-3110-08

20L

BG34-3210-08

20L

 

Ion Exchange Resins – Prepacked Column

Product name

Specification

Applicable MW

Capacity

Max pressure

Max flowrate

Application/Feature

HPDEAE-60weak anion exchange resin column

5mL
  10mL

≤200KD

≥40mg/mL

1MPa

1000cm/h

Scale-up studies for ion exchange chromatography-based protein purification processes

HPDEAE-30 weak anion exchange resin column

10ml

≤200KD

≥40mg/mL

1MPa

800cm/h

HPDEAEHL-60 weak anion exchange resin column

5mL
  10mL

≤200KD

≥90mg/mL

1MPa

1000cm/h

HPDEAEHL-30 weak anion exchange resin column

5mL
  10mL

≤200KD

≥90mg/mL

1MPa

800cm/h

HPQ-60 strong anion exchange resin column

5mL
  10mL

≤200KD

≥40mg/mL

1MPa

1000cm/h

HPQ-30 strong anion exchange resin column

5mL
  10ml

≤200KD

≥40mg/mL

1MPa

800cm/h

HPOHL-60 strong anion exchange resin column

5mL
  10mL

≤200KD

≥100mg/mL

1MPa

1000cm/h

HPQHL-30 strong anion exchange resin column

5mL
  10mL

≤200KD

≥100mg/mL

1MPa

800cm/h

HPS-60 strong cation exchange resin column

5mL
  10mL

≤200KD

≥50mg/ml

1MPa

1000cm/h

HPS-120 strong cation exchange resin column

5mL
  10mL

≤200KD

≥50mg/mL

1MPa

1000cm/h

Scale-up studies for ion exchange chromatography-based purification processes of high viscosity   solutions

CPQ-60 strong anion exchange resin column

5mL
  10mL

100KD-300KD

≥20mg/mL

1MPa

1000cm/h

Scale-up studies for ion exchange chromatography-based purification processes of high molecular   weight proteins

CPQ-30 strong anion exchange resin column

5mL
  10mL

100KD-300KD

≥20mg/mL

1MPa

800cm/h

CPOHL-60 strong anion exchange resin column

5mL

10mL

100KD-300KD

≥95mg/mL

1MPa

1000cm/h

CPQHL-30 strong anion exchange resin column

5mL
  10mL

100KD-300KD

≥95mg/mL

1MPa

800cm/h

GPDEAE-60 weak anion exchange resin column

5mL
  10mL

≥150KD

≥20mg/mL

1MPa

1000cm/h

Scale-up studies for ion exchange chromatography-based purification processes of ultra-high molecular weight proteins, viruses, virus like particles

GPDEAE-30 weak anion exchange resin column

5mL

10mL

≥150KD

≥20mg/mL

1MPa

800cm/h

GPDEAEHL-60 weak anion exchange resin column

5mL

10mL

≥150KD

≥50mg/mL

1MPa

1000cm/h

GPDEAEHL-30 weak anion exchange resin column

5mL

10mL

≥150KD

≥50mg/mL

1MPa

800cm/h

GPQ-60 strong anion exchange resin column

5mL

10mL

≥150KD

≥10mg/mL

1MPa

1000cm/h

GPQ-30 strong anion exchange resin column

5mL

10ml

≥150KD

≥10mg/mL

1MPa

800cm/h

GPQHL-60 strong anion exchange resin column

5mL

10mL

≥150KD

≥50mg/mL

1MPa

1000cm/h

GPQHL-30 strong anion exchange resin column

5mL
  10mL

≥150KD

≥50mg/mL

1MPa

800cm/h

GPS-60 strong cation exchange resin column

5mL

10mL

≥150KD

≥25mg/mL

1MPa

1000cm/h

GPS-30 strong cation exchange resin column

5mL

10mL

≥150KD

≥25mg/mL

1MPa

800cm/h

 


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