Ion exchange resin is one of the most used protein purification methods, which can separate target protein from impurities based on the difference in the isoelectric point of the proteins.
The hard gel chromatography resin developed by Bogen (A Duoning company) uses high-strength hydrophilic polyacrylate beads as matrix, and unique flexible hydrophilic modification technology to further eliminate the non-specific binding caused by the strong hydrophobic micro-surface of chromatography resins based on hard gel, which increases the recovery of active protein, improves the robustness and repeatability of process.
Bogen provides wide range of particle sizes for different purification process stages: for capture or crude feed purification, we recommend chromatography resin products with average particle size of 70 μm or 120 μm, for high-viscosity feed, consider resins with particle size of 120 μm or 200 μm; for polishing step, we recommend resins with average particle size of 40 μm or 70 μm. And please contact Bogen for more supports, if you need other chromatography resin products with non-standard particle sizes.
? Max pressure up to 10 bar
? Liner flowrate up to 1000 cm/h
? Loading capacity 60-120mg/ml (depends on application)
Function Groups
DEAE, Q, CM, S
Product Series
? HP series: use for purification of peptide and recombinant proteins
? CP series: use for purification of recombinant proteins and antibodies
? GP series: use for purification of antigens of vaccine and virus-based products
Standard particle size: 40 μm, 70 μm, 120 μm
Product Specification
Series | Applicable Molecular Weight Range | Mode | Particle Size | Max Flow Rate | Dynamic Binding Capacity (mg/mL) |
HP | ≤200KD | DEAE | 40 μm 70 μm | 1500cm/h | 90-100 (BSA) |
Q | 100-110 (BSA) | ||||
CM | 45-50 (LZM) | ||||
S | 55-60 (LZM) | ||||
CP | 100KD-300KD | DEAE | 40 μm 70 μm | 1500cm/h | 80-85 (BSA) |
Q | 95-105 (BSA) | ||||
CM | 45-50 (LZM) | ||||
S | 50-55 (LZM) | ||||
GP | ≥150KD | DEAE | 40 μm 70 μm | 1500cm/h | 50-60 (BSA) |
Q | 80-90 (BSA) | ||||
CM | 35-40 (LZM) | ||||
S | 25-30 (LZM) |
Product Features
Bogen uses a series of patented technologies to optimize the particle size, pore structure and micro-surface physical and chemical properties of polymer hard gel, achieving higher basic resolution for resin itself. Comparing to agarose resins, it provides higher resolution under same process conditions.
Strong cation exchange media resolution
Ion exchange resins: A: Bogen HPS-60; B: Product-S; C: Product-S ImpAct
Column size: 1.6cm ID x 2.5cm
Flowrate: 2mL/min
Loading volume: 5mL
Sampe: 1. Cytochrome C (1mg/mL); 2. Lysozyme (1mg/mL)
The strong cation exchange resin from Bogen can provide higher resolution under the same conditions.
Besides high resolution, the chromatography resin from Bogen provides ideal pressure-flowrate curve, which helps to maintain low back pressure during scale-up. Under increased flowrate or column height, the pressure change is relatively smooth, and much lower than agarose-based resin, which is desired for scale-up production processes.
Pressure vs. flowrate for HPS strong cation exchange resin
Bogen uses multi-scale micro-nano structure control technology to produce chromatography resin with high specific surface area porous structure. We designed resins with optimum pore size and arm space through computer simulation technology and analyzing hydraulic size of different types of biomacromolecules, achieving high dynamic binding capacity and adsorption and desorption in fast mass transfer.
Pressure vs. flow rate curves of ion exchange resins with different pore sizes
Super macro-porous resin for purification of vaccine/virus
Vaccine/virus purification is featured application of super macroporous ion-exchange resin developed by Bogen. Purifying vaccine antigen/virus vector using GPQ strong anion exchange resin or GPS strong cation exchange resin provides advantages of high loading (1-30 CVs), high purity and high yield.
Applications:
? Virus vaccine purification
? Virus-like particles (VLP) purification
? Virus vector purification, including LV、AV、 AAV
Purification of vaccine antigen
Case study
Chromatogram of model vaccine antigen purification
Resin: GPQ60 strong anion exchange resin
Column size: 1.6cm ID × 15cm
Flowrate: 5mL/min
Sample: A model vaccine antigen
Purity: Impurity removal rate >90%
Recovery rate: >80%
Ordering information
Anion Exchange Resins – HP Series
Product name | Part No. | Specification | Product name | Part No. | Specification |
HPDEAE-60 weak anion exchange resin | BG31-1100-02 | 50mL | HPDEAE-30 weak anion exchange resin | BG31-1200-02 | 50mL |
BG31-1100-04 | 500mL | BG31-1200-04 | 500mL | ||
BG31-1100-05 | 1L | BG31-1200-05 | 1L | ||
BG31-1100-07 | 10L | BG31-1200-07 | 10L | ||
BG31-1100-08 | 20L | BG31-1200-08 | 20L | ||
HPDEAEHL-60 weak anion exchange resin | BG31-1120-02 | 50mL | HPDEAEHL-30 weak anion exchange resin | BG31-1220-02 | 50mL |
BG31-1120-04 | 500mL | BG31-1220-04 | 500mL | ||
BG31-1120-05 | 1L | BG31-1220-05 | 1L | ||
BG31-1120-07 | 10L | BG31-1220-07 | 10L | ||
BG31-1120-08 | 20L | BG31-1220-08 | 20L | ||
HPQ-60 strong anion exchange resin | BG32-1110-02 | 50mL | HPQ-30 strong anion exchange resin | BG32-1210-02 | 50mL |
BG32-1110-04 | 500mL | BG32-1210-04 | 500mL | ||
BG32-1110-05 | IL | BG32-1210-05 | 1L | ||
BG32-1110-07 | 10L | BG32-1210-07 | 10L | ||
BG32-1110-08 | 20L | BG32-1210-08 | 20L | ||
HPQHL-60 strong anion exchange resin | BG32-1120-02 | 50mL | HPQHL-30 strong anion exchange resin | BG32-1220-02 | 50mL |
BG32-1120-04 | 500mL | BG32-1220-04 | 500mL | ||
BG32-1120-05 | IL | BG32-1220-05 | 1L | ||
BG32-1120-07 | 10L | BG32-1220-07 | 10L | ||
BG32-1120-08 | 20L | BG32-1220-08 | 20L |
Anion Exchange Resins – CP series
Product name | Part No. | Specification | Product name | Part No. | Specification |
CPDEAE-60 weak anion exchange resin | BG31-2100-02 | 50mL | CPDEAE-30 weak anion exchange resin | BG31-2200-02 | 50mL |
BG31-2100-04 | 500mL | BG31-2200-04 | 500mL | ||
BG31-2100-05 | 1L | BG31-2200-05 | 1L | ||
BG31-2100-07 | 10L | BG31-2200-07 | 10L | ||
BG31-2100-08 | 20L | BG31-2200-08 | 20L | ||
CPDEAEHL-60 weak anion exchange resin | BG31-2120-02 | 50mL | CPDEAEHL-30 weak anion exchange resin | BG31-2220-02 | 50ml |
BG31-2120-04 | 500mL | BG31-2220-04 | 500mL | ||
BG31-2120-05 | 1L | BG31-2220-05 | 1L | ||
BG31-2120-07 | 10L | BG31-2220-07 | 10L | ||
BG31-2120-08 | 20L | BG31-2220-08 | 20L | ||
CPQ-60 strong anion exchange resin | BG32-2110-02 | 50mL | CPQ-30 strong anion exchange resin | BG32-2210-02 | 50mL |
BG32-2110-04 | 500mL | BG32-2210-04 | 500mL | ||
BG32-2110-05 | 1L | BG32-2210-05 | 1L | ||
BG32-2110-07 | 10L | BG32-2210-07 | 10L | ||
BG32-2110-08 | 20L | BG32-2210-08 | 20L | ||
CPQHL-60 strong anion exchange resin | BG32-2120-02 | 50mL | CPQHL-30 strong anion exchange resin | BG32-2220-02 | 50mL |
BG32-2120-04 | 500mL | BG32-2220-04 | 500mL | ||
BG32-2120-05 | 1L | BG32-2220-05 | 1L | ||
BG32-2120-07 | 10L | BG32-2220-07 | 10L | ||
BG32-2120-08 | 20L | BG32-2220-08 | 20L |
Anion Exchange Resins – GP series
Product name | Part No. | Specification | Product name | Part No. | Specification |
GPDEAE-60 weak anion exchange resin | BG31-3100-02 | 50mL | GPDEAE-30 weak anion exchange resin | BG31-3200-02 | 50mL |
BG31-3100-04 | 500mL | BG31-3200-04 | 500mL | ||
BG31-3100-05 | 1L | BG31-3200-05 | 1L | ||
BG31-3100-07 | 10L | BG31-3200-07 | 10L | ||
BG31-3100-08 | 20L | BG31-3200-08 | 20L | ||
GPDEAEHL-60 weak anion exchange resin | BG31-3120-02 | 50mL | GPDEAEHL-30 weak anion exchange resin | BG31-3220-02 | 50mL |
BG31-3120-04 | 500mL | BG31-3220-04 | 500mL | ||
BG31-3120-05 | 1L | BG31-3220-05 | 1L | ||
BG31-3120-07 | 10L | BG31-3220-07 | 10L | ||
BG31-3120-08 | 20L | BG31-3220-08 | 20L | ||
GPQ-60 strong anion exchange resin | BG32-3110-02 | 50mL | GPQ-30 strong anion exchange resin | BG32-3210-02 | 50mL |
BG32-3110-04 | 500mL | BG32-3210-04 | 500mL | ||
BG32-3110-05 | 1L | BG32-3210-05 | 1L | ||
BG32-3110-07 | 10L | BG32-3210-07 | 10L | ||
BG32-3110-08 | 20L | BG32-3210-08 | 20L | ||
GPQHL-60 strong anion exchange resin | BG32-3120-02 | 50mL | GPQHL-30 strong anion exchange resin | BG32-3220-02 | 50mL |
BG32-3120-04 | 500mL | BG32-3220-04 | 500mL | ||
BG32-3120-05 | 1L | BG32-3220-05 | 1L | ||
BG32-3120-07 | 10L | BG32-3220-07 | I0L | ||
BG32-3120-08 | 20L | BG32-3220-08 | 20L |
Cation Exchange Resins – HP series
Product name | Part No. | Specification | Product name | Part No. | Specification |
HPCM-60 weak cation exchange resin | BG33-1110-02 | 50mL | HPCM-30 weak cation exchange resin | BG33-1210-02 | 50mL |
BG33-1110-04 | 500mL | BG33-1210-04 | 500mL | ||
BG33-1110-05 | 1L | BG33-1210-05 | 1L | ||
BG33-1110-07 | 10L | BG33-1210-07 | 10L | ||
BG33-1110-08 | 20L | BG33-1210-08 | 20L | ||
HPS-60 strong cation exchange resin | BG34-1110-02 | 50mL | HPS-30 strong cation exchange resin | BG34-1210-02 | 50mL |
BG34-1110-04 | 500mL | BG34-1210-04 | 500mL | ||
BG34-1110-05 | 1L | BG34-1210-05 | 1L | ||
BG34-1110-07 | 10L | BG34-1210-07 | 10L | ||
BG34-1110-08 | 20L | BG34-1210-08 | 20L |
Cation Exchange Resins – CP series
Product name | Part No. | Specification | Product name | Part No. | Specification |
CPCM-60 weak cation exchange resin | BG33-2110-02 | 50mL | CPCM-30 weak cation exchange resin | BG33-2210-02 | 50mL |
BG33-2110-04 | 500mL | BG33-2210-04 | 500mL | ||
BG33-2110-05 | 1L | BG33-2210-05 | 1L | ||
BG33-2110-07 | 10L | BG33-2210-07 | 10L | ||
BG33-2110-08 | 20L | BG33-2210-08 | 20L | ||
CPS-60 strong cation exchange resin | BG34-2110-02 | 50mL | CPS-30 strong cation exchange resin | BG34-2210-02 | 50mL |
BG34-2110-04 | 500mL | BG34-2210-04 | 500mL | ||
BG34-2110-05 | 1L | BG34-2210-05 | 1L | ||
BG34-2110-07 | 10L | BG34-2210-07 | 10L | ||
BG34-2110-08 | 20L | BG34-2210-08 | 20L |
Cation Exchange Resins – GP Series
Product name | Part No. | Specification | Product name | Part No. | Specification |
GPCM-60 weak cation exchange resin | BG33-3110-02 | 50mL | GPCM-30 weak cation exchange resin | BG33-3210-02 | 50mL |
BG33-3110-04 | 500mL | BG33-3210-04 | 500mL | ||
BG33-3110-05 | 1L | BG33-3210-05 | 1L | ||
BG33-3110-07 | 10L | BG33-3210-07 | 10L | ||
BG33-3110-08 | 20L | BG33-3210-08 | 20L | ||
GPS-60 strong cation exchange resin | BG34-3110-02 | 50mL | GPS-30 strong cation exchange resin | BG34-3210-02 | 50mL |
BG34-3110-04 | 500mL | BG34-3210-04 | 500mL | ||
BG34-3110-05 | 1L | BG34-3210-05 | 1L | ||
BG34-3110-07 | 10L | BG34-3210-07 | 10L | ||
BG34-3110-08 | 20L | BG34-3210-08 | 20L |
Ion Exchange Resins – Prepacked Column
Product name | Specification | Applicable MW | Capacity | Max pressure | Max flowrate | Application/Feature |
HPDEAE-60weak anion exchange resin column | 5mL | ≤200KD | ≥40mg/mL | 1MPa | 1000cm/h | Scale-up studies for ion exchange chromatography-based protein purification processes |
HPDEAE-30 weak anion exchange resin column | 10ml | ≤200KD | ≥40mg/mL | 1MPa | 800cm/h | |
HPDEAEHL-60 weak anion exchange resin column | 5mL | ≤200KD | ≥90mg/mL | 1MPa | 1000cm/h | |
HPDEAEHL-30 weak anion exchange resin column | 5mL | ≤200KD | ≥90mg/mL | 1MPa | 800cm/h | |
HPQ-60 strong anion exchange resin column | 5mL | ≤200KD | ≥40mg/mL | 1MPa | 1000cm/h | |
HPQ-30 strong anion exchange resin column | 5mL | ≤200KD | ≥40mg/mL | 1MPa | 800cm/h | |
HPOHL-60 strong anion exchange resin column | 5mL | ≤200KD | ≥100mg/mL | 1MPa | 1000cm/h | |
HPQHL-30 strong anion exchange resin column | 5mL | ≤200KD | ≥100mg/mL | 1MPa | 800cm/h | |
HPS-60 strong cation exchange resin column | 5mL | ≤200KD | ≥50mg/ml | 1MPa | 1000cm/h | |
HPS-120 strong cation exchange resin column | 5mL | ≤200KD | ≥50mg/mL | 1MPa | 1000cm/h | Scale-up studies for ion exchange chromatography-based purification processes of high viscosity solutions |
CPQ-60 strong anion exchange resin column | 5mL | 100KD-300KD | ≥20mg/mL | 1MPa | 1000cm/h | Scale-up studies for ion exchange chromatography-based purification processes of high molecular weight proteins |
CPQ-30 strong anion exchange resin column | 5mL | 100KD-300KD | ≥20mg/mL | 1MPa | 800cm/h | |
CPOHL-60 strong anion exchange resin column | 5mL 10mL | 100KD-300KD | ≥95mg/mL | 1MPa | 1000cm/h | |
CPQHL-30 strong anion exchange resin column | 5mL | 100KD-300KD | ≥95mg/mL | 1MPa | 800cm/h | |
GPDEAE-60 weak anion exchange resin column | 5mL | ≥150KD | ≥20mg/mL | 1MPa | 1000cm/h | Scale-up studies for ion exchange chromatography-based purification processes of ultra-high molecular weight proteins, viruses, virus like particles |
GPDEAE-30 weak anion exchange resin column | 5mL 10mL | ≥150KD | ≥20mg/mL | 1MPa | 800cm/h | |
GPDEAEHL-60 weak anion exchange resin column | 5mL 10mL | ≥150KD | ≥50mg/mL | 1MPa | 1000cm/h | |
GPDEAEHL-30 weak anion exchange resin column | 5mL 10mL | ≥150KD | ≥50mg/mL | 1MPa | 800cm/h | |
GPQ-60 strong anion exchange resin column | 5mL 10mL | ≥150KD | ≥10mg/mL | 1MPa | 1000cm/h | |
GPQ-30 strong anion exchange resin column | 5mL 10ml | ≥150KD | ≥10mg/mL | 1MPa | 800cm/h | |
GPQHL-60 strong anion exchange resin column | 5mL 10mL | ≥150KD | ≥50mg/mL | 1MPa | 1000cm/h | |
GPQHL-30 strong anion exchange resin column | 5mL | ≥150KD | ≥50mg/mL | 1MPa | 800cm/h | |
GPS-60 strong cation exchange resin column | 5mL 10mL | ≥150KD | ≥25mg/mL | 1MPa | 1000cm/h | |
GPS-30 strong cation exchange resin column | 5mL 10mL | ≥150KD | ≥25mg/mL | 1MPa | 800cm/h |
Relevant Documents
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