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MixQ Mixed Mode Resin
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MixQ mixed-mode resin

 

MixQ virus purification resin is mixed mode resin developed by Bogen (A Duoning Company) for isolation and purification of virus-based products. This product features a core-shell structure. The shell is hydrophilic polymer with defined pore size, due to large size, the virus particles flow through under exclusion effect, while the small impurities pass through the shell and enter the core. The core is polymethacrylate with rigid structure, together with n-octylamine as ligand, which absorbs impurities through ion exchange/hydrophobic interaction, thus separating viruses and impurities.

 

We provide two types of MixQ virus purification resins: MixQ700 (>MW 700,000) and MixQ400 (>MW 400,000), based on the molecular weight of size exclusion.

 

Product Features

?   Mode of virus flow-through, impurity been adsorbed

?   Impurity proteins can also be adsorbed at high salt concentrations without buffer exchange

?  The composition of the virus flow-through solution is same as feed solution, maintaining virus activity

 

MixQ mixed-mode resin 

MixQ mixed-mode resinProduct Specification

Parameter

Specification

Product Name

MixQ700S virus purification resin

MixQ700L virus purification resin

Average particle size

70 μm

120 μm

Matrix

Polyacrylate hard gel

Size exclusion molecular weight

700 KDa

Max Pressure

1 MPa

Dynamic capacity

≥10mg/mL

PH Stability

Resistant to 0.5 M NaOH + 30% IPA cleaning

Storage

4-30 ℃ (20% ethanol) or 5-10 mM NaOH


Parameter

Specification

Product Name

MixQ400S virus purification resin

MixQ400L virus purification resin

Average particle size

70μm

120 μm

Matrix

Polyacrylate hard gel

Size exclusion molecular weight

400 KDa

Max Pressure

1MPa

Dynamic capacity

≥10mg/mL

PH stability

Resistant to 0.5 M NaOH + 30% IPA cleaning

Storage

4-30 ℃ (20% ethanol) or 5-10 mM NaOH

 

Case Study 1

 

Chromogram of vaccine antigen purification process by MixQ700Lvirus purification resin

 

MixQ mixed-mode resin
                                                  

Column size: 1.6cm ID × 10cm

Process preparation: concentrate 15 X

Chromatography process parameters:

Flowrate: 2 mL/min

Loading volume: 20 mL

Collect flow-through fraction

 

 

MixQ mixed-mode resin

Sample

Volume

Virus   titer (TCID50/0.1mL)

A280-IGG   (μg/mL)

Concentrated   original sample

30   mL

7.8

21026

Collected   purified sample

40   mL

7.8

4042

 Electrophoresis results of virus purification process by MixQ mixed mode resin

 

Case Study 2

MixQ mixed-mode resin

Adenovirus vector purification

 

Resin: MixQ700L virus purification resin

Column size: 1mL

Flowrate: 1mL/min

Sampe: Model vaccine antigen

 

MixQ Mixed Mode Resins - for virus purification

Product Name

Part No.

Specification

Product Name

Part No.

Specification

MixQ700L virus purification resin

BG65-1010-02

50mL

MixQ700S virus purification resin

BG65-1110-02

50mL

BG65-1010-04

500mL

BG65-1110-04

500mL

BG65-1010-05

1L

BG65-1110-05

1L

BG65-1010-07

10L

BG65-1110-07

10L

BG65-1010-08

20L

BG65-1110-08

20L

MixQ400L virus purification resin

BG65-2010-02

50mL

MixQ400S virus purification resin

BG65-2110-02

50mL

BG65-2010-04

500mL

BG65-2110-04

500mL

BG65-2010-05

1L

BG65-2110-05

1L

BG65-2010-07

10L

BG65-2110-07

10L

BG65-2010-08

20L

BG65-2110-08

20L


MixQ Mixed Mode Resin Column - for virus purification

Product Name

Specification

Size exclusion molecular weight

Capacity

Max Pressure

Max Flow rate

Application/Feature

MixQ700L virus purification column

5mL

700KDa

≥10mg/mL

1MPa

1000cm/h

Scale-up study of virus purification process

10mL

MixQ700S virus purification column

5mL

700KDa

≥10mg/mL

1MPa

1000cm/h

10mL

MixQ400L virus purification column

5mL

400KDa

≥10mg/mL

1MPa

1000cm/h

10mL

MixQ400S virus purification column

5mL

400KDa

≥10mg/mL

1MPa

1000cm/h

10mL

 

 

 


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  • E-Mail: marketing@duoningbio.com
  • Address: Building 30, No. 1525 Minqiang Road, Songjiang District, Shanghai
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