The unique mixing method of DuoWave? wave bioreactor provides a gentle growth environment for cells. The newly designed process control software has various functions and a simple and intuitive interface, which is easy to get familiar. The working volume of DuoWave? wave bioreactor ranges from 500 ml to 25 L, which is suitable for the seed train expansion, inoculum preparation, culture process development of various types of cells and fast preparation of laboratory samples.
Main functions
DuoWave? wave bioreactor adopts a new modular design, which consists of four modules: base, tray, I/O and server. It is easy to install, operate and saves space.
? The base module is designed with functions of temperature measurement, rocking and mixing, weighing and exhaust gas heating. After installing the tray, it can be started by setting the LCD touch screen that comes with the base.
? The tray includes two specifications of 20L and 50L, and adopts a dual-zone heating plate design, which can realize independent temperature control of two cell culture bags.
? The I/O module integrates optical pH, DO electrodes, gas mixing and high-precision peristaltic pump functions, and users can easily realize process parameter feedback control.
? The server module includes DuoWave? control software and computer, through which users can perform process control, audit trail, data recording, four-level authority management, batch report generation and other operations. DuoWave? wave bioreactor system complies with GMP and 21 CFR Part 11 requirements.
Applications
Depending on the mode of operation, DuoWave? wave bioreactors can be applied to
? Seed Expansion
? Fed-Batch
? Perfusion
? Microcarrier
Depending on different applications, DuoWave? wave bioreactors can be applied to
? Antibody and recombinant proteins
? Vaccine
? Gene therapy
? Cell therapy
? Other biologics or macro molecular
Mass transfer and mixing performance
DuoWave? wave bioreactor system not only has excellent mass transfer and mixing performance, but also brings extremely low shear force and suitable growth environment for cell culture due to its unique non-sparging aeration and non-stirring design.
Component configuration and part number
Module | Main module model | Configuration | Parameters |
Base | Base
| Temperature control module | Double-plate independent temperature control, continuously adjustable from RT to 42°C, PID closed-loop control, water-cooled bottom plate can be customized. |
Rocking mixing module | Rocking angle is continuously adjustable from 0-12°, and the speed is continuously adjustable from 0-23 rpm | ||
Weighing Module | 0 – 50 kg, can cascade with peristaltic pump | ||
2 exhaust gas heaters | 80 ± 10 °C automatic constant temperature | ||
Tray | Tray20
| 2 separate temperature measuring and heating plates | Compatible with DuoWave? 3 L, 10 L, 20 L cell culture bags |
Tray50
| 2 separate temperature measuring and heating plates | Compatible with DuoWave? 22 L, 50 L cell culture bags | |
I/O Module | I/O module
| Two Watson-Marlow 114D variable speed peristaltic pumps; Optical DO and pH sensors Gas mixing device | Peristaltic pump flow rate range 0-5mLPM（13#）, 0-22mLPM（14#）, 0-80mLPM（16#）, 0-200mLPM（25#）. The measure range of DO and pH are 0-200% and 5-9, respectively. The gas flowrate range of gas 0-1 LPM, with CO2 range of 0-20% and O2 range of 0-90%. |
Server Module | Microsoft Surface
| Microsoft Surface Pro7 Built-in DuoWave? control software | i5, 8G+128G, standard + Pro original keyboard |
Laptop
| Dell laptop Built-in DuoWave? control software | i5, 8G or above RAM, 512G or above SSD | |
computer
| Dell PC Built-in DuoWave? control software | i5 , 8G memory, 512G or above solid state drive; Windows 10 or Windows 11 Professional Edition |
Dimension | Weight | |
Base | 470*560*264 mm | 27.7 kg |
20L Tray | 792*535*62 mm | 10.7 kg |
50L Tray | 904*688*74 mm | 16.9 kg |
20L Tray（w/ cooling） | 792*535*65 mm | 15.4 kg |
50L Tray（w/ cooling） | 904*688-77 mm | 21.9 kg |
20L top cover | 832*549*274 mm | 4.9 kg |
50L top cover | 904*688*307 mm | 6.3 kg |
I/O module | 310*418*185 mm | 10.8 kg |
pH optical fiber | 1.5 m | |
DO optical fiber | 1.5 m |
Base | Performance parameter |
Rocking speed | 0-40 rpm |
Rocking angle | 0-12° |
Weighing range | 0-50 kg |
Weighing accuracy | 10 g |
Temperature electrode | Pt100 |
Temperature measurement range | 0-80 °C |
Temperature measurement accuracy | ±0.1 °C |
Temperature control range | 10°C below room temperature (w/ cooling tray), room temperature to 45°C |
Temperature control accuracy | ±0.2 °C |
I/O Module | Performance parameter |
Gas flowrate range | Air 0.3 L/min CO2 0.3 L/min*20% O2 0.3 L/min |
Gas flowrate accuracy | 0.5% of full-scale |
Fast filling gas flow rate | 3 LPM |
CO2 control range | 0-20% |
CO2 measurement accuracy | ±0.2% |
CO2 control accuracy | ±0.5% |
O2 control range | N2 mode 0-80% & Air mode 21%-80% |
O2 measurement accuracy | 2% |
O2 control accuracy | 0.5% |
pH measurement range | pH 4.5-pH 8.5 |
pH control range | pH 6.0-pH 8.0 |
pH measurement accuracy | ±0.05 pH, within ±0.25 pH of the calibration value ±0.1 pH, ±0.25 to ±0.5 pH from calibration value |
pH control accuracy | ±0.05 pH |
DO measurement range | 0-250% |
DO control range | 0-100% |
DO measurement accuracy | ±5% |
Peristaltic Pump | Performance parameter |
Number of peristaltic pumps | 2* Watson-Malow 114DV |
Flowrate range | 0-200 mL/min |
Pump accuracy | 10% of total volume |
Compatible tubing sizes | 13# (1/32 in-5/32 in) 14# (1/16 in-3/16 in) 16# (1/8 in-1/4 in) 25# (3/16 in-5/16 in) |
DuoWave? Cell Culture Bag
Features of DuoFilm-003 film material
DuoFilm-003 is a 5-layer coextruded film that withstands the harshest physical requirements while exhibiting excellent cell culture compatibility.
? The outer layer is low-density polyethylene (LDPE), which has excellent chemical stability and elongation.
? The middle layer is ethylene-vinyl alcohol copolymer EVOH, which exhibits excellent barrier effect on gases, odors, solvents, etc., while maintaining flexibility and strength properties
? Liquid contact layer is ultra low density polyethylene (ULDPE) for excellent biocompatibility and low extractable levels while providing a clean, inert surface with no anti-blocking additive ingredients.
We provide DuoWave? cell culture bags with various sizes and configurations for different bioprocessing applications.
Configuration | Description |
Tube material | Optional silicone tube or weldable tube |
pH/DO | Optional configurations with or without optical electrodes |
Volume | Optional 3L, 10L, 15L, 20L, 22L, 50L |
Sampling port | Luer sampling port |
Film material | DuoFilm?- 003 |
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Relevant Documents
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